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KMID : 1189420230360010001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2023 Volume.36 No. 1 p.1 ~ p.20
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Anti-inflammatory Effects of Tanghwajitong-san through Inhibition of NF-κB and MAPK
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Ko Min-Jung
Jee Seon-Young
Hwangbo Min
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Abstract
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Objectives : The purpose of this study was to evaluate the anti-inflammatory effect of Tanghwajitongsan(THJTS) through inhibition of NF-κB and MAPK.
Methods : We evaluated cell survival rate by MTT assay, NO production by nitrite content in the culture mediu m. We quantified TNF-α, IL-1β, IL-6 and PGE 2 of the cultured supernatant by ELISA. And we evaluated the effect of THJTS on protein expression of NF-κB, MAPK, iNOS and COX-2 by Western blot analysis. THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-infl ammatory cytokines and PGE 2 . Also, THJTS ameliorates LOX, PGN and FLA-activated alterations in protein expres sion of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein expression of COX-2.
Results : THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-inflammatory cytokines and PGE 2 . Also, THJTS ameliorates LOX, PGN and FLA-activat ed alterations in protein expression of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein ex pression of COX-2.
Conclusions : This study can provide scientific evidence for the anti-inflammatory effects and underlying mech anisms of THJTS.
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KEYWORD
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Tanghwajitong-san, Angelicae Gigntis Radix, Rhei Rhizoma, Inflammation, NF-κB, MAPK
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